Recombinant Human CDH10 protein, Fc-tagged
Recombinant Human CDH10(Glu23-Ala613) fused with Fc tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Glu23 & Gly55
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is typically 0.1-0.4 μg/mL.Optimal dilutions should be determined by each laboratory for each application.
Predicted Molecular Mass: 92.7 (monomer) kDa;SDS-PAGE: 100-120 kDa, reducing conditions.
<0.01 EU per 1 mug of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.