Recombinant full-length human CDK7 and CCNH were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tags.
CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression. CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle.
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
The specific activity of CDK7/CyclinH1 was determined to be 56nmol/min/mg as per activity protocol.
CDK7 ~41~43 kDa, and CCNH ~39 kDa
The purity of CDK7/CCNH was determined to be >70% by densitometry.
Kinase Assay; WB
1 year at -70 centigrade
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.