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Home > Products > Recombinant Proteins > Recombinant Human CDK7/CCNH Active protein, His-tagged

Recombinant Human CDK7/CCNH Active protein, His-tagged

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"CDK7/CCNH" Related Products.
By Species
Human(1)
By Source
By Tag
His(1)
Cat.No.:
CDK7-CCNH-313H
Product Overview:
Recombinant full-length human CDK7 and CCNH were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tags.
Description:
CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression. CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle.
Source:
Insect cell
Species:
Human
Tag :
His
Form:
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Bio-activity:
The specific activity of CDK7/CyclinH1 was determined to be 56nmol/min/mg as per activity protocol.
Molecular Mass:
CDK7 ~41~43 kDa, and CCNH ~39 kDa
Purity:
The purity of CDK7/CCNH was determined to be >70% by densitometry.
Applications:
Kinase Assay; WB
Stability:
1 year at -70 centigrade
Storage:
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:
0.1 μg/μL


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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