Recombinant Human CHD4 protein, His-tagged

• Cat.No.: CHD4-2434H
• Product Overview: Recombinant Human CHD4 fused with His tag was expressed in Insect cells. CHD4 is a full-length cDNA coding for the human Mi-2 beta isoform.

Specification

• Description: CHD4 is a member of a family of alleged chromodomain helicase-DNA-binding proteins. Biochemically, CHD4 is a component of the nucleosome transformation and deacetylase (NuRD) complex that takes part in transcription regulation. Autoantibodies targeting the CHD4 are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Moreover, anti-CHD4 antibodies are related to dermatomyositis (frequency up to 31%) and have a great positive predictive value for this type of disease subset. Anti-CHD4 are the only defined myositis-specific autoantibodies clearly focused to a nuclear target. An additional slightly outstanding feature of Mi-2 antibodies relates to their frequency in children, which is similar to that in adults.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: CHD4 is supplied in 20mM HEPES buffer pH-8.0 and 500mM NaCl.
• Molecular Mass: 221,298 Dalton
• Purity: Greater than 95% as determined by SDS-PAGE.
• Applications: Western blot with myositis sera or monoclonal anti-hexa-His-tag antibody. Coating concentration: 0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: CHD4 chromodomain helicase DNA binding protein 4 [ Homo sapiens ]
• Official Symbol: CHD4
• Synonyms: CHD4; chromodomain helicase DNA binding protein 4; chromodomain-helicase-DNA-binding protein 4; Mi 2b; Mi2 BETA; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA; DKFZp686E06161
• Gene ID: 1108
• mRNA Refseq: NM_001273
• Protein Refseq: NP_001264
• MIM: 603277
• UniProt ID: Q14839
• Chromosome Location: 12p13
• Pathway: Signaling events mediated by HDAC Class I, organism-specific biosystem
• Function: ATP binding; ATP-dependent DNA helicase activity; DNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; metal ion binding; nucleotide binding; protein binding; transcription factor binding; zinc ion binding

Reviews

12/10/2022

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02/27/2022

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10/22/2020

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Q&As

How does CHD4 interact with other components of the chromatin remodeling complex?

CHD4 collaborates with the NuRD complex in chromatin remodeling.

What role does CHD4 play in gene regulation?

CHD4 regulates gene transcription via chromatin accessibility.

What is the primary role of CHD4 in chromatin remodeling?

CHD4 alters chromatin structure, aiding in DNA accessibility.

How do genetic variations in CHD4 affect gene expression?

Genetic changes in CHD4 affect gene expression and cellular function.

What is the impact of altered CHD4 activity on cancer development?

Altered CHD4 activity is associated with cancer progression.

How does CHD4 contribute to DNA damage repair?

It remodels chromatin for efficient DNA repair.

What potential therapeutic applications arise from targeting CHD4 in cancer treatment?

Targeting CHD4 offers potential cancer treatment strategies by affecting cell growth.