Recombinant human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.
Predicted N Terminal:
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH.
The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.