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Home > Products > Recombinant Proteins > Recombinant Human CHEK1 Protein, GST-tagged

Recombinant Human CHEK1 Protein, GST-tagged

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By Species
Human(13)
Mouse(5)
Rat(4)
Zebrafish(1)
By Tag
Flag(1)
GST(4)
His(4)
His & GST(1)
N/A(1)
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Cat.No.:
CHEK1-229H
Product Overview:
Recombinant human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.
Source:
Baculovirus-Insect Cells
Species:
Human
Tag:
GST
Predicted N Terminal:
Met
Form:
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH.
Bio-activity:
The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
Molecular Mass:
The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>95 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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