Recombinant human CHI3L2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CHI3L2, also known as chitinase-3-like protein 2, belongs to chitinase protein family. It is secreted by synovial fibroblasts, chondrocytes, and TGFb/IL4 stimulated monocyte derived macrophages. It has been proposed as a potential biomarker for osteoarthritis.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
41.9kDa (372aa)40-57KDa (SDS–PAGE under reducing conditions)
YKLVCYFTNW SQDRQEPGKF TPENIDPFLC SHLIYSFASI ENNKVIIKDK SEVMLYQTIN SLKTKNPKLK ILLSIGGYLF GSKGFHPMVDSSTSRLEFIN SIILFLRNHN FDGLDVSWIY PDQKENTHFT VLIHELAEAF QKDFTKSTKE RLLLTAGVSA GRQMIDNSYQ VEKLAKDLDFINLLSFDFHG SWEKPLITGH NSPLSKGWQD RGPSSYYNVE YAVGYWIHKG MPSEKVVMGI PTYGHSFTLA SAETTVGAPA SGPGAAGPITESSGFLAYYE ICQFLKGAKI TRLQDQQVPY AVKGNQWVGY DDVKSMETKV QFLKNLNLGG AMIWSIDMDD FTGKSCNQGP YPLVQAVKRSLGSLLEHHHH HH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)