Recombinant human EFEMP1 cDNA (26 – 443 aa, derived from BC010456) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHENLYFQGGEFELSPQDSEEPDSYTECTDGYEWDPDSQHCRDVNECLTIPEACKGEM KCINHYGGYLCLPRSAAVINDLHGEGPPPPVPPAQHPNPCPPGYEPDDQDSCVDVDECAQALHDCRPSQDCHNLP GSYQCTCPDGYRKIGPECVDIDECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRSCVDVNECDMGAPCEQRCFN SYGTFLCRCHQGYELHRDGFSCSDIDECSYSSYLCQYRCINEPGRFSCHCPQGYQLLATRLCQDIDECESGAHQC SEAQTCVNFHGGYRCVDTNRCVEPYIQVSENRCLCPASNPLCREQPSSIVHRYMTITSERSVPADVFQIQATSVY PGAYNAFQIRAGNSQGDFYIRQINNVSAMLVLARPVTGPREYVLDLEMVTMNSLMSYRASSVLRLTVFVGAYTF
1. May be used for in vitro EFEMP2 protein mediated cancer cell growth regulation study with this protein as either coating matrix protein or as soluble factor.2. May be used for EFEMP2 protein – protein interaction assay.3. May be used as enzymatic substrate for various proteases.4. Potential biomarker protein for diagnosis of malignant pleural mesothelioma..5. May be used for specific antibody production.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.