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Home > Products > Recombinant Proteins > Recombinant Human EGFR protein, Avi/His-tagged, Biotinylated

Recombinant Human EGFR protein, Avi/His-tagged, Biotinylated

"EGFR" Related Products.
Product Name:
Recombinant Human EGFR protein, Avi/His-tagged, Biotinylated
Product Overview:
Recombinant Human EGFR(Leu 25 - Ser 645) fused with Avi/His tag at C-terminal was expressed in HEK293.
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors; EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2–7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point
Tag :
Predicted N Terminal:
Leu 25
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass:
Has a calculated MW of 41.3 kDa. As a result of glycosylation, The protein migrates as 60-70 kDa under reducing (R) condition and 60-70 kDa under non-reducing (NR) condition on SDS-PAGE gel.
Protein length:
Leu 25 - Ser 645
AA Sequence:
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitute at 200 μg/mL in sterile deionized water.

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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