Recombinant Human EIF4A2, fused with N-His tag, was expressed in E. coli.
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5"-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
20mM Tris.Cl, 50mM NaCl, 20% Glycerol, pH8.0.
48.3kDa(including fusion tag)
422aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80°C and avoid repeated freeze-thaw cycles