Active Recombinant Human EP300 protein

• Cat.No.: EP300-42H
• Product Overview: Recombinant Human EP300 catalytic domain (aa 1284-1673) was expressed in E. coli.

Specification

• Description: Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
• Source: E. coli
• Species: Human
• Form: Liquid. In 50mM TRIS/HCl, pH 8.0, containing 0.1mM EDTA, 10% glycerol.
• Bio-activity: ≥250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis(2-nitrobenzoic acid)).
• Molecular Mass: 45.1 kDa
• Purity: Partially purified by single-step affinity chromatography and gel filtration.
• Applications: Can be used to study p300 kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example, [3H]-Acetyl CoA.
• Storage: Long Term Storage: -80 centigrade

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; histone acetyltransferase p300; KAT3B; p300; p300 HAT; E1A-binding protein, 300kD; E1A-associated protein p300; RSTS2
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q09472
• Chromosome Location: 22q13.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell cycle, organism-specific biosystem

Reviews

09/22/2022

It has consistently demonstrated outstanding results, consistently producing clear and sharp bands, allowing for accurate and reliable protein detection and quantification.

03/09/2021

Its stability and purity make it an ideal candidate for studying protein structures at a high resolution, providing crucial insights into the organization and function of the EP300 protein.

07/07/2016

I highly recommend the EP300 protein for its exceptional performance in Western blot analysis.

Q&As

How does EP300 impact developmental abnormalities?

EP300 is crucial for embryonic development, and mutations in the EP300 gene can lead to developmental abnormalities. Understanding its role is essential for potential interventions.

How does EP300 contribute to neurodegenerative disorders?

EP300 is involved in neuronal function and survival. Dysregulation of EP300 has been observed in neurodegenerative diseases like Alzheimer's and Parkinson's.

Are there experimental treatments targeting EP300 in neurodegenerative disorders?

Research is underway to explore drugs that modulate EP300 activity for potential therapeutic interventions in neurodegenerative disorders, but clinical applications are still in the early stages.

Are there any ongoing clinical trials targeting EP300 in cancer treatment?

Yes, there are clinical trials exploring the therapeutic potential of targeting EP300 in various cancer types, focusing on its role in controlling cell proliferation and survival.

Are there drugs targeting EP300 in cardiovascular diseases?

While there are no specific drugs targeting EP300 approved for cardiovascular diseases, ongoing research aims to identify compounds that can modulate its activity for therapeutic purposes.