Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human ERBB2 Protein, His & GST-tagged

Recombinant Human ERBB2 Protein, His & GST-tagged

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By Tag
Fc(6)
Fc-His(1)
GST(6)
His(14)
His & GST(1)
Human(2)
N/A(4)
None(1)
StrepII(1)
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Cat.No.:
ERBB2-176H
Product Overview:
Recombinant cytoplasmic domain (Lys 676-Val 1255) of human ErbB2 (NP_004439.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source:
Baculovirus-Insect Cells
Species:
Human
Tag:
His & GST
Predicted N Terminal:
Gln 26
Form:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % gly, pH 8.0, 5%~8% trehalose and mannitol.
Bio-activity:
No Kinase Activity
Molecular Mass:
The recombinant human ErbB2 (676-1255)/GST chimera consists of 817 amino acids and predictes a molecular mass of 92 kDa. It migrates as an approximately 100 KDa band in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>75 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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