Recombinant Human EYA2 protein, His-tagged

• Cat.No.: EYA2-18H
• Product Overview: Recombinant Human EYA2(244-514aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: EYA2, also known as eyes absent homolog 2, is a member of tyrosine phosphatase family that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). This protein promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
• Molecular Mass: 33.2 kDa (295aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDLERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EYA2 eyes absent homolog 2 (Drosophila) [ Homo sapiens ]
• Official Symbol: EYA2
• Synonyms: EYA2; eyes absent homolog 2 (Drosophila); eyes absent (Drosophila) homolog 2; eyes absent homolog 2; EAB1; MGC10614
• Gene ID: 2139
• mRNA Refseq: NM_005244
• Protein Refseq: NP_005235
• MIM: 601654
• UniProt ID: O00167
• Chromosome Location: 20q13.1
• Function: hydrolase activity; magnesium ion binding; protein binding; protein tyrosine phosphatase activity

Reviews

11/17/2020

I am confident that the EYA2 protein will unravel crucial insights into molecular mechanisms, greatly enhancing the significance and impact of my scientific investigations.

03/15/2019

the manufacturer's commitment to providing excellent technical support adds an additional layer of confidence to my choice of the EYA2 protein.

09/17/2016

By relying on the EYA2 protein, I can approach my research with unwavering confidence in its quality and the unparalleled support extended by its manufacturer.

Q&As

Are there any therapeutic implications for targeting EYA2 protein?

Targeting EYA2 protein could have therapeutic potential in cancer treatment, either by inhibiting its function or modulating its activity.

How does EYA2 protein contribute to cell proliferation?

EYA2 protein is involved in regulating the cell cycle and promoting cell proliferation, which has implications for cancer development and progression.

What are the implications of EYA2 protein in drug resistance?

EYA2 protein has been linked to drug resistance in cancer cells, making it an important factor to consider in developing effective treatment strategies.

What are the potential effects of EYA2 protein on cell differentiation?

EYA2 protein has been shown to influence cell differentiation processes, potentially impacting tissue development and regeneration.

How does EYA2 protein affect DNA repair mechanisms?

EYA2 protein has been shown to interact with DNA repair proteins, influencing the efficiency of DNA repair processes.