Recombinant human FGF8a (NP_149355.1) (Gln23-Arg204) was expressed with an initial Met.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5, 5% ~ 8% trehalose and mannitol.
Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 μg/mL.
The recombinant human FGF8a consists 183 amino acids and predicts a molecular mass of 21.3 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.