Recombinant Human GAK(1-396 aa) fused with GST tag at N-terminal was expressed in Insect cells.
Cyclin-G-associated kinase (GAK) is a serine/threonine protein kinase and was originally identified as a protein that is associated with cyclin G. However, neither CDK5 nor GAK kinase activity is activated by cyclin G nor does GAK affect CDK5 kinase activity. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
1 year at -80 centigrade from the date of shipment
Store at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.