Recombinant Human GLA(Met1-Leu429) fused with His tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-galactopyranoside. The specific activity is >1,100 pmol/min/μg.
Predicted Molecular Mass: 46 kDa;
SDS-PAGE: 45-55 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.