Recombinant human GRB2 cDNA (2 – 217 aa) fused with T7-His-TEV cleavage site Tag at N-terminal was expressed in E. coli.
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
MASMTGGQQMGRGHHHHHHENLYFQGGSEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFI PKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVV KFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGAC HGQTGMFPRNYVTPVNRNV
1. May be used for in vitro protein mediated Wnt / b-catenin pathway regulation study in tumor cell transformation or ES cell differentiation with this protein as either coating matrix protein or soluble factor.2. May be used for GRB2 protein-protein interaction assay.3. Enzymatic substrate for various proteases.4. As antigen for specific antibody production.
Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.