Recombinant Human HEMK1 fused with GST tag at N-terminal was expressed in Insect cells.
HEMK1 belongs to the HemK methyltransferase family, members of which are found in genomes from bacteria to humans. HEMK1 methylates the glutamine residue in the GGQ motif of HMRF1L, a human mitochondrial release factor, and plays an important regulatory role in translation termination process.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.