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Recombinant Human HGF protein

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Cat.No.:
HGF-195H
Product Overview:
Recombinant Human HGF( HGF protein isoform 1 (comprising the signal peptide, and alpha and beta chains)) was expressed in HEK293.
Source:
HEK293
Species:
Human
Form:
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Bio-activity:
The ED50 of HGF is typically 20 - 40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line.
Molecular Mass:
Under reducing conditions HGF migrates as a broad band between 80 and 110 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified HGF that has a predicted monomeric molecular mass of 79.7 kDa.
Purity:
>95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue.
Storage:
Lyophilised products should be stored at 2 to 8 centigrade. Following reconstitution short-term storage at 4 centigrade is recommended and longer-term storage of aliquots at -18 to -20 centigrade.
Repeated freeze thawing is not recommended.
Reconstitution:
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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