IL12A & IL12B-1254H
Recombinant Human IL12A & IL12B Protein, His-Fc & Flag-Fc-tagged
Recombinant p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant human IL27B, (NP_005746.2) (Met1-Lys229) was fuse
Predicted N Terminal:
Arg 23 & Arg 21
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
The recombinant human IL12A&IL27B comprises 905 (447+458) amino acids and has a calculated molecular mass of 102 (50.7+ 51.3) KDa. The apparent molecular mass of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
(59.8+33.3) % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.