Recombinant Human KRT18, transcript variant 1, fused with His tag at N-terminal was expressed in E. coli.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
>50 ug/mL as determined by microplate BCA method