Recombinant human LIFR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
LIFR, also known as leukemia inhibitory factor receptor, is the receptor for leukemia inhibitory factor, a pleiotropic cytokine affecting the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
90.5kDa (798aa)100-150KDa (SDS-PAGE under reducing conditions.)
ADPQKKGAPH DLKCVTNNLQ VWNCSWKAPS GTGRGTDYEV CIENRSRSCY QLEKTSIKIP ALSHGDYEIT INSLHDFGSS TSKFTLNEQNVSLIPDTPEI LNLSADFSTS TLYLKWNDRG SVFPHRSNVI WEIKVLRKES MELVKLVTHN TTLNGKDTLH HWSWASDMPL ECAIHFVEIRCYIDNLHFSG LEEWSDWSPV KNISWIPDSQ TKVFPQDKVI LVGSDITFCC VSQEKVLSAL IGHTNCPLIH LDGENVAIKI RNISVSASSGTNVVFTTEDN IFGTVIFAGY PPDTPQQLNCETHDLKEIICSWNPGRVTALVGPRATSYTLVESFSGKYVRLKRAEAPTNESYQLLFQMLPNQEIYNFTLN AHNPLGRSQS TILVNITEKV YPHTPTSFKV KDINSTAVKL SWHLPGNFAK INFLCEIEIK KSNSVQEQRN VTIKGVENSSYLVALDKLNP YTLYTFRIRC STETFWKWSK WSNKKQHLTT EASPSKGPDT WREWSSDGKN LIIYWKPLPI NEANGKILSY NVSCSSDEETQSLSEIPDPQ HKAEIRLDKN DYIISVVAKN SVGSSPPSKI ASMEIPNDDL KIEQVVGMGK GILLTWHYDP NMTCDYVIKW CNSSRSEPCLMDWRKVPSNS TETVIESDEF RPGIRYNFFL YGCRNQGYQL LRSMIGYIEE LAPIVAPNFT VEDTSADSIL VKWEDIPVEE LRGFLRGYLFYFGKGERDTS KMRVLESGRS DIKVKNITDI SQKTLRIADL QGKTSYHLVL RAYTDGGVGP EKSMYVVTKE NSHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)