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Home > Products > Recombinant Proteins > Recombinant Human MAP3K5 protein, MYC/DDK-tagged

Recombinant Human MAP3K5 protein, MYC/DDK-tagged

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By Species
Human(13)
Mouse(4)
Zebrafish(1)
By Tag
Flag(1)
GST(3)
His(3)
His & T7(1)
MYC/DDK(1)
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Cat.No.:
MAP3K5-20H
Product Overview:
Recombinant Human MAP3K5 fused with MYC/DDK tag at C-terminal was expressed in HEK293.
Description:
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Source:
HEK293
Species:
Human
Tag :
MYC/DDK
Form:
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Mass:
154.4 kDa
Purity:
> 80% as determined by SDS-PAGE and Coomassie blue staining
Notes:
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Concentration:
>50 ug/mL as determined by microplate BCA method


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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