Recombinant human METAP1 (NP_055958.2) (His52-Phe386) was expressed with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol, 5%~8% trehalose and mannitol.
The recombinant human METAP1 consists 347 amino acids and predicts a molecular mass of 39.3 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.