Human MS4A1 / CD20 Protein, TrxA Tag, Ile 141 - Ser 188 (Accession # NP_068769) was produced in E.coli cells.
Predicted N Terminal:
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Measured by its binding ability in a functional ELISA. Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Human CD20 Protein with linear range of 0.08-2.5 μg/mL when detection with Anti-TrxA mouse mab.
This protein carries a trxA at the N-terminus, and has a calculated MW of 22.7 kDa. The reducing protein migrates as 25 kDa in SDS-PAGE.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.