Recombinant Human NCL protein, His-tagged

• Cat.No.: NCL-07H
• Product Overview: Recombinant Human NCL containing the C-terminal section of the human nucleolin and missing the N-terminal histone-binding part of nucleolin, fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Description: Nucleolin (NCL) which is a eukaryotic nucleolar phosphoprotein, involved in the synthesis and maturation of ribosomes. Nucleolin is the key nucleolar protein of growing eukaryotic cells. NCL is found linked with intranucleolar chromatin and pre-ribosomal particles. NCL induces chromatin decondensation by binding to histone H1. Nucleolin is assumed to have a role in pre-rRNA transcription and ribosome compilation. Nucleolin may also have a role in the process of transcriptional elongation. Nucleolin is located primarily in the dense fibrillar regions of the nucleolus. The Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: NCL is supplied in 20mM HEPES pH-7.3, 600mM NaCl, 0.3mM Tris(2-carboxyethyl)phosphine (TCEP) and 25% glycerol.
• Molecular Mass: 55,162 Dalton
• Purity: Greater than 80.0% as determined by SDS-PAGE.
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: NCL nucleolin [ Homo sapiens ]
• Official Symbol: NCL
• Synonyms: NCL; nucleolin; C23; FLJ45706; FLJ59041
• Gene ID: 4691
• mRNA Refseq: NM_005381
• Protein Refseq: NP_005372
• MIM: 164035
• UniProt ID: P19338
• Chromosome Location: 2q12-qter
• Pathway: Aurora B signaling, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem; Pathogenic Escherichia coli infection, conserved biosystem; Regulation of Telomerase, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional activation, organism-specific biosystem
• Function: DNA binding; RNA binding; nucleic acid binding; nucleotide binding; protein C-terminus binding; protein binding; telomeric DNA binding

Reviews

11/28/2022

The company provides one-stop service, from the purchase of products to the completion of experiments in one go.

07/14/2022

The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.

12/26/2021

The staff had a good attitude, actively communicated with me and solved many technical problems.

Q&As

Where is NCL primarily located?

NCL is mainly localized in the nucleus.

What diseases is NCL involved in?

NCL is closely related to the study of diseases such as nasopharyngeal cancer and endometrial cancer.

What are the components of NCL?

The NCL consists of three parts: a nuclear localization signal, an RNA binding domain, and RGG or GAR structural domains.

What are the effects of overexpressing NCL?

Overexpression of NCL increases PCV2 viral load in cells.

What biological processes are NCL involved in?

NCL is involved in the regulation of various physiological stages such as cell proliferation, differentiation, and embryogenesis.