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Home > Products > Recombinant Proteins > Recombinant Human NOTCH4 protein, His-tagged

Recombinant Human NOTCH4 protein, His-tagged

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By Species
Human(5)
Mouse(2)
Rat(3)
By Source
By Tag
His(3)
Cat.No.:
NOTCH4-120H
Product Overview:
Recombinant Human NOTCH4(Asp1715~Gly2001) fused with His tag at N-terminal was expressed in E. coli.
Description:
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Source:
E. coli
Species:
Human
Tag :
His
Form:
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass:
33.3kDa
Protein length:
Asp1715~Gly2001
Endotoxin:
<1.0EU per 1µg (determined by the LAL method)
Purity:
> 95%
Applications:
Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage:
Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution:
Reconstitute in PBS or others.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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