Human TrkC / NTRK3 Protein (Human TrkC, His Tag) Cys 32 - Thr 429 (Accession # AAH13693.1) was produced in human 293 cells (HEK293).
Predicted N Terminal:
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Measured by its ability to inhibit NT-3-induced proliferation of BaF-TrkB-BD mouse pro-B cells transfected with TrkB. The ED50 for this effect is typically 0.01 - 0.1 ug/mL in the presence of 40 ng/mL of recombinant human NT-3.
Human TrkC, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 45.6 kDa. The reducing protein migrates as 80-90 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.