|Product Overview:||Recombinant Human PARK2 was expressed in Insect cells.|
|Description:||The E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene) is an essential part of the cellular machinery that participates in the removal of damaged mitochondria. Mutations in PARK2 are known to cause a form of Parkinson's disease known as autosomal recessive juvenile Parkinson's disease (AR-JP), and the mechanisms by which defective Parkin ligase contributes to the dopaminergic cell death in this disease is an area of intense investigation.|
|Form:||X mg/ml (X μM) in 25 mM Tris pH 8.5, 200 mM NaCl, 0.03% Brij35, 10% (v/v) Glycerol, 5 mM TCEP|
|Bio-activity:||Reaction conditions will need to be optimized for each specific application. As supplied, Parkin has negligible E3 ligase activity as determined by the lack of autoubiquitination in an in vitro assay. Parkin ligase activity is greatly enhanced by phosphorylation with PINK1 kinase or by the addition of low concentrations of phosphorylated Ubiquitin.|
|Molecular Mass:||52 kDa|
|Purity:||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
|Gene Name:||PARK2 parkinson protein 2, E3 ubiquitin protein ligase (parkin) [ Homo sapiens ]|
|Synonyms:||PARK2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); Parkinson disease (autosomal recessive, juvenile) 2, parkin; E3 ubiquitin-protein ligase parkin; AR JP; E3 ubiquitin ligase; parkin; PDJ; parkin 2; parkinson disease protein 2; parkinson juvenile disease protein 2; PRKN; AR-JP; LPRS2;|
|Pathway:||Adaptive Immune System, organism-specific biosystem; Alpha-synuclein signaling, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem;|
|Function:||PDZ domain binding; acid-amino acid ligase activity; chaperone binding; kinase binding; ligase activity; metal ion binding; protein binding; protein kinase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding;|
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