Recombinant Human PNPLA2 fused with MYC/DDK tag at C-terminal was expressed in HEK293.
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
>50 ug/mL as determined by microplate BCA method