Recombinant Human PPME1(2-end) fused with GST tag at N-terminal was expressed in Insect cells.
Protein phosphatase methylesterase 1 (PPME1) conducts the demethylation of the catalytic subunit of protein phosphatase 2A (PP2A) and leads to inactivation of the phosphatase. It is demonstrated that PPME1 binds directly to the active site of PP2A and evicts the manganese ions required for the phosphatase activity. PPME1 is also reported to protect ERK activity in human malignant glioma.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
1 year at -70 centigrade from the date of shipment
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.