Human PTPRC, transcript variant 1, derived in Human HEK293T cell line.
Human HEK293T cell line
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.