Recombinant Human RHOC fused with a histidine tag in the amino-terminus was expressed in E. coli.
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.
The biological activity of His-RhoC is determined from its ability to catalyze the exchange of GDP for GTP.
RhoC biochemistry; RhoC GTPase assays; RhoC nucleotide exchange assays; RhoC binding studies
Reconstituted in distilled water to 1 mg/ml