Recombinant Human ROR1 protein, His-tagged

• Cat.No.: ROR1-1813H
• Product Overview: Human ROR1 (165-305, Frizzled domain), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 165 - Asp 305 (Accession # Q01973).

Specification

• Description: Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Glu 165
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 21 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Protein length: AA Glu 165 - Asp 305
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ROR1 receptor tyrosine kinase like orphan receptor 1 [ Homo sapiens (human) ]
• Official Symbol: ROR1
• Synonyms: NTRKR1; dJ537F10.1; neurotrophic tyrosine kinase; receptor-related 1
• Gene ID: 4919
• mRNA Refseq: NM_001083592.1
• Protein Refseq: NP_001077061.1
• UniProt ID: Q01973