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Home > Products > Recombinant Proteins > Recombinant Human SMAD3 protein, GST-tagged

Recombinant Human SMAD3 protein, GST-tagged

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GST(4)
His(6)
His/MBP(2)
Cat.No.:
SMAD3-295H
Product Overview:
Recombinant Human SMAD3(C-terminal SXS deletion) fused with GST tag at N-terminal was expressed in E. coli.
Description:
SMAD3 is a direct mediator of transcriptional activation by the TGF receptor. The activity of SMAD3 is regulated by the TGF receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGF stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor. SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGF response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.
Source:
E. coli
Species:
Human
Tag :
GST
Form:
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Molecular Mass:
84 kDa
Purity:
> 85%
Applications:
Western Blot
Stability:
1 year at -70 centigrade from the date of shipment.
Storage:
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:
0.1μg/μl


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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