Recombinant human THRB cDNA (461aa, derived from BC106930) fused with 31 aa T7/His/TEV at N-terminal was expressed in E. coli.
0.1 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFELTPNSMTENGLTAWDKPKHCPDREHDWKLVGMSEACLHRKSHSER RSTLKNEQSSPHLIQTTWTSSIFHLDHDDVNDQSVSSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYR CITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEEN REKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFS HFTKIITPAITRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGG LGVVSDAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPK LLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED
1. As soluble active recombinant protein, may be used for in vitro THRB protein functional study of thyroid hormone pathway regulation.2. As active protein, may be used for EMSA based DNA / protein binding assay.3. As protein substrate for kinase or ubiquitin enzymatic assay.4. As immunogen for specific antibody production.
Keep at -20centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.