Recombinant Human TIMP3 protein, His-tagged
Recombinant Human TIMP3(Cys24-Pro211) fused with His tag at N-terminal was expressed in E. coli.
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
Lyophilized from a 0.2 µm filtered PBS solution (pH 7.4).
Purity: >90%, as determined by SDS-PAGE under reducing conditions and visualized by Coomassie blue staining.
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc. Before use, heat the WBC control at 95 centigrade for 5 minutes. Spin down the sample and load 5 µL per lane onto SDS-PAGE gel.
Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5~1.0 mg/mL with sterile deionized water or PBS.