Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Recombinant Human TIMP3 protein, His-tagged

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Fc(2)
His(6)
N/A(1)
Cat.No.:
TIMP3-852H
Product Overview:
Recombinant Human TIMP3(Cys24-Pro211) fused with His tag at N-terminal was expressed in E. coli.
Description:
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
Source:
E. coli
Species:
Human
Tag:
His
Form:
Lyophilized from a 0.2 µm filtered PBS solution (pH 7.4).
Molecular Mass:
23.4 kDa
Purity:
Purity: >90%, as determined by SDS-PAGE under reducing conditions and visualized by Coomassie blue staining.
Applications:
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc. Before use, heat the WBC control at 95 centigrade for 5 minutes. Spin down the sample and load 5 µL per lane onto SDS-PAGE gel.
Storage:
Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5~1.0 mg/mL with sterile deionized water or PBS.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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