Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human TINAGL1 protein, His-tagged

Recombinant Human TINAGL1 protein, His-tagged

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Human(6)
Mouse(3)
Rat(2)
Zebrafish(1)
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By Tag
His(5)
Cat.No.:
TINAGL1-238H
Product Overview:
Recombinant Human TINAGL1(Ala22-Met464) fused with His tag at C-terminal was expressed in CHO.
Description:
TINAGL1 (Tubulointerstitial nephritis antigen-like 1; also TIN-ag-RP, AZ-1 and Lipocalin 7) is a 54-57 kDa glycoprotein member of the peptidase C1 family of molecules. Mature human TINAGL1 contains an SMB (somatomedin B) domain, one vWFC domain, and a nonenzymatic peptidase C1A region. Mature human TINAGL1 shares 90% amino acid (aa) identity with mouse TINAGL1. Alternative splicing generates additional isoforms that either lack a portion of the vWFC domain or consist of a portion of the peptidase domain with a 38 aa insertion and flanked by substitutions. TINAGL1 is secreted by renal tubule epithelium, zona glomerulosa cells, vascular smooth muscle, and striated muscle cells. It is also expressed in mouse decidual endometrium and blastocysts. During pregnancy it binds to Laminin-1 and Integrins alpha 5 and beta 1 and plays a role in supporting fertility. In the vasculature, TINAGL1 localizes to basal laminae along adrenocortical sinusoidal capillaries where it binds to fibronectin and collagen and supports adrenocortical cell adhesion.
Source:
CHO
Species:
Human
Tag :
His
Predicted N Terminal:
Ala22
Form:
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Cultrex Mouse Laminin I is coated at 5 µg/mL, Recombinant Human TINAGL1 binds with an ED50 = 1-6 ng/mL.
Molecular Mass:
Predicted Molecular Mass: 52 kDa
SDS-PAGE: 47-61 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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