Recombinant Human TNFAIP3 fused with MYC/DDK tag at C-terminal was expressed in HEK293.
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
>50 ug/mL as determined by microplate BCA metho