Recombinant Human TNFRSF12A Protein, Fc-tagged
Recombinant human TNFRSF12A isoform 1 (Q9NP84-1) extracellular domain (Glu 28-Trp 79) was fused with the Fc region of human IgG1 at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A, The EC50 of human Fc-TNFRSF12A is 0.07-0.15 μg/ml.
The recombinant human TNFRSF12A/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 313 amino acids and has a calculated molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTNFRSF12A/Fc monomer is approximately 37 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.