Biotinylated Human HVEM / TNFRSF14, Fc Tag (HV4-H82F1) is expressed from human HEK293 cells. It contains AA Leu 39 - Val 202 (Accession # NP_003811).
Predicted N Terminal:
Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Measured by its binding ability in a functional ELISA against immobilized Human TNF-beta (1 μg/well). The linear range is 0.6-10 μg/mL.
This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 45.8 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.