Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human TNFRSF14 Protein, Fc & Avi-tagged, Biotinylated

Recombinant Human TNFRSF14 Protein, Fc & Avi-tagged, Biotinylated

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Fc(4)
Fc & Avi(1)
Fc-His(4)
His(5)
N/A(1)
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Cat.No.:
TNFRSF14-625H
Product Overview:
Biotinylated Human HVEM / TNFRSF14, Fc Tag (HV4-H82F1) is expressed from human HEK293 cells. It contains AA Leu 39 - Val 202 (Accession # NP_003811).
Source:
HEK293
Species:
Human
Tag:
Fc & Avi
Predicted N Terminal:
Leu 39
Form:
Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Bio-activity:
Measured by its binding ability in a functional ELISA against immobilized Human TNF-beta (1 μg/well). The linear range is 0.6-10 μg/mL.
Molecular Mass:
This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 45.8 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
Endotoxin:
Less than 1.0 EU per μg by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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