Recombinant Human TNFRSF6B(Val 30 - His 300) fused with a human IgG1 Fc tag at the C-terminus was expressed in HEK293.
Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) is also known as Decoy Receptor 3 (DcR3), Decoy receptor for Fas ligand and M68, which belongs to the tumor necrosis factor receptor superfamily. TNFRSF6 is a soluble protein which contains four TNFR-Cys repeats. Unlike most of the other members of TNFR superfamily, TNFRSF6 is a soluble protein which contains no transmembrane domain. TNFRSF6B acts as a decoy receptor that competes with death receptors for ligand binding. The protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 56.4 kDa. DTT-reduced Protein migrates as 64 kDa in SDS-PAGE due to glycosylation.
Val 30 - His 300
Less than 1.0 EU per μg of the rh TNFRSF6B / DcR3 Fc Chimera by the LAL method.
>85% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).