Recombinant human TNFSF8, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
TNFSF8, also known as tumor necrosis factor ligand superfamily member 8, is a type II membrane protein belonging to the TNF superfamily. It is expressed on the cell surface of activated T cells, B cells, and monocytes. CD30/TNFRSF8 ligation by TNFSF8 mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
20.7kDa (181aa)18-40KDa (SDS–PAGE under reducing conditions.)
ADPQRTDSIP NSPDNVPLKG GNCSEDLLCI LKRAPFKKSW AYLQVAKHLN KTKLSWNKDG ILHGVRYQDG NLVIQFPGLY FIICQLQFLVQCPNNSVDLK LELLINKHIK KQALVTVCES GMQTKHVYQN LSQFLLDYLQ VNTTISVNVD TFQYIDTSTF PLENVLSIFL YSNSDHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>85% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)