Recombinant full length human tag-free Tau-441 was co-expressed with DYRK1A in E. coli cells. The Tau-441 was phosphorylated by DYRK1A in vivo and in vitro prior to the final chromatography purification.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
>95% by densitometry
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.