Recombinant Human WDR77 fused with His tag at N-terminal was expressed in E. coli.
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.
25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Stable for at least 3 months from receipt of products under proper storage and handling conditions.
>50 ug/mL as determined by microplate BCA method