Recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012)(AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4.The EC50 of of biotinylated DPP4 is 0.52-1.22 μg/ml. 2. Measured by its binding ability in a funct
The recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.