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Recombinant MERS-CoV (NCoV / Novel coronavirus) S Protein, His-tagged

Cat.No.: S-599V
Product Overview: Recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012)(AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus.
Source: Baculovirus-Insect Cells
Species: MERS-CoV
Tag: His
Predicted N Terminal: Tyr 18
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4.The EC50 of of biotinylated DPP4 is 0.52-1.22 μg/ml. 2. Measured by its binding ability in a funct
Molecular Mass: The recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: S S protein [ Middle East respiratory syndrome coronavirus ]
Official Symbol: S
Synonyms: spike glycoprotein; S protein
Gene ID: 14254594
mRNA Refseq:
Protein Refseq:
MIM:
UniProt ID:

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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