Recombinant mouse ANTXR2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
ANTXR2, also known as anthrax toxin receptor 2, is a widely expressed anthrax toxin receptor family protein. The main functional domain of this protein is an extracellular integrinlike von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site, through which it adheres selectively to collagen type IV and laminin. This protein and the related protein ATR/TEM8 serve as receptors for the protective antigen of Bacillus anthracis.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
31.9kDa (295aa)28-40KDa (SDS–PAGE under reducing conditions.)
QAQEQPSCKK AFDLYFVLDK SGSVANNWIE IYNFVHQLTE RFVSPEMRLS FIVFSSQATI ILPLTGDRYK IGKGLEDLKA VKPVGETYIH EGLKLANEQI QNAGGLKASS IIIALTDGKL DGLVPSYAEN EAKKSRSLGA SVYCVGVLDF EQAQLERIAD SKDQVFPVKG GFQALKGIIN SILAQSCTEI LELSPSSVCV GEKFQVVLTG RAVTSISHDG SVLCTFTANS TYTKSEKPVS IQPSSILCPA PVLNKDGETL EVSISYNDGK SAVSRSLTIT ATECTNGLEH HHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)