Recombinant Mouse CD276 Protein, His-tagged
Recombinant mouse CD276 (NP_598744.1) (Met1-Phe244) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 5%~8% trehalose and mannitol.
The recombinant mouse CD276 comprises 227 amino acids and has a predicted molecular mass of 24.9 kDa. The apparent molecular mass of the protein is approximately 40.9 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.