Recombinant mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
The recombinant mouse CA2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>96 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.