Recombinant Mouse Dhh was expressed in E. coli.
Desert hedgehog protein (DHH) is a member of the Hedgehog family which encodes signaling molecules that play an important role in regulating morphogenesis. It is predicted to be made as a precursor that is auto-catalytically cleaved; the N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH may be involved in both male gonadal differentiation and perineurial development. DHH binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). It induces steroidogenicfactor 1(SF1), which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules.
Lyophilized after extensive dialysis against PBS.
ED50 <20 μg/ml, measured by its ability to induce alkaline phosphatase production by CCL-226 cells.
20.1 kDa, observed by reducing SDS-PAGE.
176 amino acids
IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASGPAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTERCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH
YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNHIHVSVKADNS LAVRAGG
< 0.2 EU/μg, determined by LAL method.
> 98% as analyzed by SDS-PAGE and HPLC.
Lyophilized recombinant Mouse Desert Hedgehog (DHH) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Mouse Desert Hedgehog (DHH) should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
Reconstituted in ddH2O or PBS at 100 μg/ml.