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Home > Products > Recombinant Proteins > Recombinant Mouse Dkk2 protein, His-tagged

Recombinant Mouse Dkk2 protein, His-tagged

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By Species
Human(10)
Mouse(7)
Rat(2)
Zebrafish(1)
By Tag
Fc/His(1)
His(6)
N/A(1)
Cat.No.:
Dkk2-194M
Product Overview:
Recombinant Mouse Dkk2(Ser26-Ile259) fused with Leu35Pro substitution and His tag at C-terminal was expressed in E. coli.
Description:
Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators. Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain mediates Dkk-2 binding activities, and its interaction with LRP beta propellers has been mapped. The 226 aa, ~35 kDa mature mouse Dkk-2 shares 99%, 96%, 96%, 96% and 94% aa identity with rat, human, dog, horse and cow Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos. Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6. When LRP6 is over-expressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling. However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes. Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling. In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye, although it is expressed as early as periimplantation in mice. Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite down-regulating Wnt antagonism in both cases. Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition. In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density. Dkk-2-deficient mice are blind, exhibiting faulty differentiation of corneal epithelium and ectopic blood vessels in the periocular mesenchyme.
Source:
E. coli
Species:
Mouse
Tag :
His
Predicted N Terminal:
Ser26
Form:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity:
Measured in a competitive binding assay. When Recombinant Mouse Kremen‑1 is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse Dkk-2 inhibits 50% binding of biotinylated Recombinant Human Dkk-1 (200 ng/mL) at the concentration range of 0.25-1.5 µg/mL.
Molecular Mass:
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 29-35 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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