Recombinant Mouse F7 Protein, His-tagged
Recombinant mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Val 25 & Ala 42
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA.Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his. The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/ml.
The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.